![]() The chromatogram is thereby “stored” for future analysis with each fraction assigned a timestamp of the LC/MS analysis. The effluent is split post column with a small portion directed to the Advion ESI Chip ® for ionization and LC/MS analysis while the remainder of the split effluent is collected into a multi-well plate. Users can couple any conventional HPLC system to the TriVersa NanoMate ® LESA ® for MS analysis (optional RAD or UV detectors can also be utilized). Mode 2 is ideally suited for researchers interested in metabolite identification, biomarker analysis, and protein characterization or any application that requires more time for analysis than what is allowed during conventional liquid chromatography MS analysis. ![]() In addition, excess LC eluate can be utilized for fraction collection to create a physical storage of the LC/MS experiment and allow re-analysis of fractions of interest as identified by the online LC/MS run. ![]() Mode 2 was developed to operate the system as a nano-ESI ion source for LC/MS applications.
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